Introduction: Agrochemical researches are critical for meeting needs in food and fiber production, as well as disease vector control. The agriculture industry is stressful and there is evidence that persistent stress can contribute to the onset or progression of mental health problems. Aim of Work: To study the prevalence, sources and level of stress using three indicator tools, and the possible relationship between stress and mental well-being, among a group of agrochemical researchers in Egypt. Materials and Methods: A face-to-face interview was performed and the professional life stress (PLSS), the Health and Safety Executive (HSE) indicator tool, in addition to the 12-Items General Health Questionnaire (GHQ-12) were distributed to 94 agrochemical researchers. Results: Out of 94 questionnaires; 69 replied (with response rate 73.4%). Stress was not a problem among 47.8% of the participants, whereas 49.3% have a Moderate range of stress. Psychologically distressed personnel constituted 21.7% of the total participants. Change, demand and lack of authorities support were the predominant sources of stress among the studied population. Demand and relationships were statistically significant among the problematic stressed personnel than the others. Psychological distress was significantly higher among problematic stress group compared to the other two groups. Conclusion and Recommendations: Stress reported by the studied agrochemical researchers was mostly of Moderate and Non-problematic level. Demand and relationships were significant stressors for problematic stress, and psychological distress. The current study may be considered as the first step in implementing a stress management program to be followed by targeted interventions as group discussions and awareness sessions, organizational change and other aspects of stress prevention/ management programs.