The marine microbiome consists of diverse microorganisms in ocean environments that are essential for nutrient cycling, ecosystem health, and global biogeochemical. This study focused on isolating and identifying a new bacterial strain collected from the Red Sea. The strain has the ability to produce antifungal compound as a bioactive secondary metabolite. One of six bacterial isolates from Gulf of Suez showed antagonistic activities against different fungi pathogens. The potent Bacillus strain (H19) was identified by MALDI-TOF as Bacillus Cereus. The pathogen fungi (Aspergillus flavus, Penicillium glaprun, Aspergillus niger, mucor circinelloid and Candida albicans) were all antagonistically determined by H19. The strain (H19) demonstrated the largest zone of inhibition against Candida albicans (29±0.1mm). Gas chromatography-mass spectrometry (GC-MS) analysis of the 2:1 methanol:chloroform extracts of the bacterial culture supernatant from strain H19 revealed the presence of two predominant bioactive compounds: Bis(2-ethylhexyl) phthalate and Octadecanoic acid, 2,3-dihydroxypropyl ester. These compounds were identified as possessing significant antimicrobial activity.