The rate of Candida- induced infections has been increasing significantly. The magnitude of the problem is aggravated by the emerging antifungal resistance among various Candida species. Our study aimed to assess the antifungal susceptibility profiles of Candida isolates causing infections at Ain Shams University Hospitals from 2018 to 2022. A cross-sectional observational study of all cases of positive growth of Candida and antifungal susceptibility testing was performed using VITEK 2 compact automated system.  Out of 342 specimens, Candida albicans (C. albicans) was the most common species (40.4%). Candida non albicans (CNA) was predominating (59.6%) and was mainly composed of C. tropicalis (36.3%) and C. parapsilosis (8.8%). Among total Candida isolates, 24 (7%) were resistant to Flucytosine, 20 (5.8%) were resistant to Amphotericin B as well as Caspofungin, 12 (3.5%) were resistant to Fluconazole, 5 (1.5%) were resistant to both Micafungin and Voriconazole. Candida non albicans which express decreased susceptibility to antifungals have been emerging as a serious cause of infection among hospitalized patients. Resistance was common to Flucytosine, Amphotericin B and Caspofungin, with low resistance rate to Fluconazole, Micafungin and Voriconazole.