Introduction: Small-bowel ulcers have many causes and nonsteroidal anti-inflammatory drugs (NSAIDs) represent the most common causes of drug-induced intestinal injury. Peripheral blood mononuclear cell (PBMC) therapy plays an important role in tissue healing and repair, and their priming with activated platelet supernatant (APS) has been known to augment their efficacy through induction of angiogenesis and tissue regeneration.
Aim of the Work: The research aimed to study the influence of APS-primed mononuclear cells on the ileal ulcers induced by indomethacin in adult male albino rats.
Materials and Methods: 45 adult male albino rats were used, 10 of them were used as donors and the other 35 were used as recipient rats which were divided into; group I (acted as a control) and group II were subdivided into subgroup II-A (animals received a single oral dose of 25 mg/kg indomethacin and were sacrificed 6 hours after ulcer induction), subgroup II-B (animals treated by intravenous injection of APS-primed PBMCs after induction of ileal ulcer and were sacrificed after 2 weeks). Finally, the ileum was obtained and processed for histological and immunohistochemical studies.
Results: Subgroup II-A (ulcer induction) revealed marked histological structural changes as focal damage, atrophy of villi, desquamated cells, shrunken irregular cells, darkly stained nuclei, vacuolated cytoplasm, cellular infiltration and congestion of blood vessels. APS-primed mobilized PBMCs therapy induced significant improvement in the histological structure of the ileum (Subgroup II-B).
Conclusion: APS-primed mononuclear cells exerted a significant ameliorative effect on the ileum after ulcer induction by indomethacin.