Introduction: Carbofuran, a broad-spectrum carbamate insecticide (endocrine disruptor) affects thyroid glands. Selenium-NPs is effective as selenite as a trace element for thyroid gland functioning while being less hazardous.
Aim of wWork: The aim is to examine probable protecting effect of Cys Se-NPs on carbofuran induced rat thyroid glands damage.
Materials and Methods: Forty adult male albino rats were divided randomly to four groups; control group, carbofuran group: received 2.4 mg/kg bw carbofuran, carbofuran & Cys Se-NPs group: received carbofuran as previous group in addition to 0.3 mg/kg bw Cys Se-NPs and finally Cys Se-NPs group: received Cys Se-NPs. Serum analysis of T3 and, T4 hormones and tissue GSH and MDA markers were evaluated. Thyroid sections of 5 μm thickness were prepared for H&E stains, Masson's trichrome stains and detection of Ki67 antibodies immunohistochemical stain. Other thyroid specimens were processed for examination by transmission electron microscope.
Results: Carbofuran group significantly revealed reduction in GSH and both T3, T4 and increase level of MDA. Thyroid follicles were highly disorganized and destructed, with follicular cells stratification and significant increase in epithelial thickness. Exfoliated cells were detected in the colloid with reduction in its volume. Carbofuran & Cys Se-NPs group revealed significant rise in GSH and T3, T4 and reduction in MDA level. Histological and ultrastructure abnormalities were improved significantly. Ki67 index was reduced by 3.69% compared to 62.4% in carbofuran group.
Conclusions: Cys Se-NPs improved the changes induced by the toxic effects of carbofuran which can be a promising therapy against ROS produced by the carbofuran.