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342062

Effect of Apitoxin on Alveolar Bone Alterations in Induced Diabetic Rats (An Animal Study)

Article

Last updated: 01 Jan 2025

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Tags

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Abstract

Introduction: Diabetes mellitus (DM) is a metabolic disorder caused by deficient insulin secretion or action or both. Among the main complications of DM is altered bone metabolism. Apitoxin, or honey bee venom (BV), has recently been suggested to have beneficial therapeutic effects against various diseases, including diabetes.
Objective: Evaluation of the possible therapeutic effect of BV in ameliorating diabetes-induced alterations in rat's alveolar bone.
Materials and Methods: 18 adult male rats, about 3-4 months age, were utilized. 6 rats served as control (Group I), DM was induced in the remaining rats through streptozotocin injection. Diabetic rats were randomly divided into: Group II (treated with vehicle), Group III (treated with BV). All rats were sacrificed after 1 month, mandibles were examined histologically, by scanning electron microscopy and by RT-PCR to assess osteoprotegerin (OPG) and interleukin (IL)-17 genes' expression.
Results: Histologically, group I showed a normal alveolar bone structure, while following diabetes induction, in group II, degenerative changes were noticed. Treatment with BV (group III) resulted in a relatively well restored histological structure of alveolar bone. Scanning electron microscopic results revealed a smooth buccal cortical plate in group I, while in group II, a generalized destruction was noticed. However; in group III, the bone surface demonstrated slight roughness. Statistical results presented a significant decrease in OPG gene expression in both group II and III than in group I. However; OPG gene expression was significantly increased in group III in comparison to group II. A significant increase occurred also in IL-17 gene expression in groups II and III. On the other hand, this expression significantly decreased in group III than in group II.
Conclusion: These results could suggest that BV treatment could ameliorate diabetes-induced alveolar bone changes; this could be attributed to the anti-osteoclastogenic and anti-inflammatory effects of BV.

DOI

10.21608/ejh.2022.146295.1707

Keywords

apitoxin, Bee venom, diabetic rats, IL-17, Osteoprotegerin

Authors

First Name

iman

Last Name

aboushady

MiddleName

-

Affiliation

oral biology department, faculty of dentistry, Cairo university, Egypt

Email

iman.aboushady@dentistry.cu.edu.eg

City

cairo

Orcid

0000-0003-3284-1878

First Name

dina

Last Name

farag

MiddleName

badawy

Affiliation

Oral Biology Department, Faculty of Dentistry, Cairo University

Email

dina.farag@dentistry.cu.edu.eg

City

cairo

Orcid

0000-0002-8856-8906

Volume

46

Article Issue

4

Related Issue

46113

Issue Date

2023-12-01

Receive Date

2022-06-22

Publish Date

2023-12-01

Page Start

1,870

Page End

1,879

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_342062.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=342062

Order

23

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

Effect of Apitoxin on Alveolar Bone Alterations in Induced Diabetic Rats (An Animal Study)

Details

Type

Article

Created At

23 Dec 2024