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The Therapeutic Effect of Honey Bee Venom versus Mesenchymal Stem Cells on Submandibular Salivary Glands of Streptozotocin-Induced Diabetic Rats

Article

Last updated: 23 Dec 2024

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Abstract

Introduction: Diabetes mellitus is a serious disease of worldwide concern. Therapeutic bee venom (BV) and bone marrow mesenchymal stem cells (BMSCs) were widely applied in various diseases.
Aim of the Work: This study was conducted to compare the effect of BV and BMSCs on the submandibular salivary glands of streptozotocin (STZ)-induced diabetic rats.
Materials and Methods: In the ongoing study, 40 male rats were used. Five rats were utilized for BMSCs isolation and culture, 5 rats served as the control group, however the remaining 30 rats were distributed as follow: untreated diabetic group; received a single intraperitoneal (IP) dose of STZ, BV treated diabetic group; diabetic rats received a daily IP dose of 0.5 mg/kg BV for 4 weeks, and BMSCs treated diabetic group; diabetic rats received a single IV injection of BMSCs. All animals were euthanized after a month, the submandibular salivary glands were dissected and evaluated by histological, immunohistochemical and quantitative Real-Time Polymerase Chain Reaction (qRT- PCR) examinations. Finally, all obtained data were statistically analyzed.
Results: Regarding control group the salivary gland architecture was normal, while the diabetic group revealed degenerative glandular changes. Both diabetic treated groups showed improved histological pictures, however; improvement was more obvious in the BMSCs treated group in comparison to the BV treated one. Regarding the diabetic group, a significantly increased inducible nitric oxide synthase
(iNOS) immunoexpression and Hemeoxygenase-1 (HO-1) expression were noticed. However, following BV and BMSCs treatments, a significantly decreased iNOS immunoreactivity and overexpression of HO-1 gene were noticed.
Conclusion: Both BMSCs and BV treatments ameliorated degenerative effects of diabetes, yet; BMSCs exhibited a more significant therapeutic effect than BV.

DOI

10.21608/ejh.2022.118315.1635

Keywords

Bee venom, heme oxygenase-1 gene, inducible nitric oxide synthase, stem cells, streptozotocin

Authors

First Name

Heba

Last Name

Abd Elwahab

MiddleName

Mahmoud

Affiliation

oral biology, faculty of dentistry, Cairo University, Cairo

Email

heba.mahmoud@bue.edu.eg

City

Cairo

Orcid

-

First Name

Hala

Last Name

Zaatar

MiddleName

Salah El-Deen

Affiliation

Professor of Oral Biology, Oral Biology Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.

Email

hala.zaatar@bue.edu.com

City

Cairo

Orcid

-

First Name

Sara

Last Name

El Moshy

MiddleName

-

Affiliation

Lecturer of Oral Biology, Faculty of Dentistry, Cairo University, Cairo, Egypt.

Email

sarah.mahmoud@dentistry.cu.edu.eg

City

Cairo

Orcid

0000-0002-2860-8523

First Name

Dina

Last Name

Sabry

MiddleName

-

Affiliation

Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

Email

dinasabry@kasralainy.edu.eg

City

Cairo

Orcid

-

First Name

Iman

Last Name

Aboushady

MiddleName

-

Affiliation

PhD, Associate Professor, Oral Biology department, Faculty of Dentistry, Cairo University, 11 Al Saraya, Al Manial, Giza Governorate, 11553 Egypt

Email

eimoshady@hotmail.com

City

Cairo

Orcid

0000-0003-3284-1878

Volume

46

Article Issue

3

Related Issue

44342

Issue Date

2023-09-01

Receive Date

2022-02-04

Publish Date

2023-09-01

Page Start

1,097

Page End

1,107

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_227928.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=227928

Order

4

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

The Therapeutic Effect of Honey Bee Venom versus Mesenchymal Stem Cells on Submandibular Salivary Glands of Streptozotocin-Induced Diabetic Rats

Details

Type

Article

Created At

23 Dec 2024