Abstract
Background: Diabetes mellitus (DM) is a metabolic disorder that has many complications like cardiovascular diseases, diabetic nephropathy and unhealed diabetic skin wounds.
Aim of the work: To evaluate the possible curative effect of insulin and platelet rich plasma (PRP) on diabetic skin wounds induced in adult male albino rats.
Materials and Methods: 50 adult male albino rats, with an average body weight 150-200 gm, were divided into (control and experimental groups). The control groups (20 rats) that were equally subdivided into two groups (control I: normal healthy rats with intact skin and control II: diabetic rats with non-treated skin wounds managed with local intradermal saline injections 500 μL once daily at wounds margins). Experimental groups (30 rats) that were equally further subdivided into three groups; Insulin treated group I: diabetic rats with local intradermal insulin injections 0.5 U/500 μL once daily at wounds margins. PRP treated group II: diabetic rats with local intradermal PRP injections 500 µl as a single dose at wounds margins. Combined treated group III: diabetic rats with local intradermal combined PRP and insulin injections with 2 hours intervals. Each group in the control and experimental groups was further subdivided into 2 subgroups A and B according to the time of scarification after 14 and 21 days post wounds induction respectively.
Results: The experimental groups showed significant improvement in wound closure, epidermis regeneration and increased collagen deposition as compared to non -treated control group II. The insulin and combined treated groups showed increased collagen deposition as compared to PRP treated group, however, the combined group showed the best results.  Conclusion: Local combined treatment with insulin and PRP showed a rapid rate of diabetic wounds closure.