Salinity reduces agricultural production's yield and quality, which results in major global economic losses. Jasmonic acid and glycine betaine are well known for their significant roles in facilitating physiological and metabolic processes in plants and for boosting their resistance to different abiotic stressors. The objective of the study was to lessen the negative impacts of saline stress on tomato plants. The field experiments were conducted in the Experimental Station of Desert Research Center in Ras Sudr, South Sinai Governorate, Egypt, during the summer 2020 and 2021 seasons to investigate the effect of jasmonic acid (0, 5, and 10 mM) and glycine betaine (0, 50, and 100 mM) on tomato plant growth, photosynthetic pigments, and content of sucrose, malondialdehyde, and proline, as well as yield and fruit quality. Based on the results, plant growth characteristics, photosynthetic pigments, yield, and its various characteristics, including fruit diameter, fruit number and fruit weight per plant were stimulated by applying either 5 mM of jasmonic acid or 50 mM of glycine betaine, and both together. In addition, treatment with jasmonic acid and/or glycine betaine increased the fruit firmness, total soluble solids, total titratable acidity, and ascorbic acid contents. Sucrose, malondialdehyde, and proline contents were markedly decreased with the application of jasmonic acid and/or glycine betaine treatment. Consequently, given that jasmonic acid and glycine betaine help tomato plants which are stressed by salinity, these treatments may be used as an alternate strategy for growing plants under salinity conditions.