Zinc is vital for the growth of plants. Globally, zinc insufficiency is a widespread micronutrient shortfall in areas of agriculture, resulting in decreased yield and nutritional quality of agricultural plants. It is necessary for plants to grow normally and productively. The fieldwork was conducted in the 2021–2022 seasons at the Experimental Farm of Desert Research Center, Baloza Research Station, North Sinai Governorate. The experiments aimed to evaluate the effects of three different sources of zinc fertilizer as ZnSO₄, Zn-EDTA and ZnO NPs at 0, 50, 100 and 150 ppm on growth, yield, nutrients contents of thyme plants. As well as the elemental composition of thyme plants using qualitative energy-dispersive X-ray microanalysis (EDX) system attached with scanning electron microscopy (SEM) as EDX-SEM. Treatments were arranged in a split plot design with three replicates. The results showed that, the best treatment was Zn NPs at 100 mg l^-1 that gave the maximum growth, components of yield, and nutrient concentrations of thyme plants. In this work, it was found that thyme plants contained a major concentration of C and O while N presented as moderate concentration, but Si, P, K, Ca, Cu and Zn presented trace concentration.