A field trial was conducted throughout the 2021 and 2022 seasons on "Flame seedless" grapevine cultivated in sandy soil. Grapevines were irrigated from a well with a drip irrigation system at an orchard in El-Farafrah Oasis in the New Valley Governorate, Egypt. The aim of this study is to investigate the effects of foliar spray with three rates of mepiquat chloride (0, 200, and 300 ppm) and five different concentrations of anti-transpiration (0, 10 cm/l glycerol, 15 cm/l glycerol, 2 and 4 cm/l Green Miracle) and their combinations on vegetative characteristics, yield and fruit quality of Flame seedless grapevine. Obtained results showed that untreated plants with mepiquat chloride compined with Green Miracle at 4 cm/l decreased shoot length, number of leaves/shoot and leaf area. Meanwhile, mepiquat chloride at 300 ppm compined with Green Miracle at 4 cm/l had statistically the highest productivity measurements and improving cluster and berry quality of Flame seedless grapevines under the circumstances of this study.