Sugarcane is one of the most important commercial crops in Egypt which has a long-life cycle and a high-water requirement in general. Sugarcane production and growth are negatively impacted by water constraint, especially during the key water requirement period. This study compared nine sugarcane genotypes to the commercial genotype GT.54-9 (Saccharum spp.) in terms of cane yield, sugar yield, and juice quality related to physio-biochemical attributes including chlorophyll (Chl) Soil Plant Analysis Development (SPAD index), Relative Water Content (RWC), and proline content as a biochemical constituent. Under three levels of drought stress (87.5, 75, and 62.5% of reference crop evapotranspiration (ET0). In the severe water scarcity situations (62.5% of ET0). The results showed that the most promising genotypes (G.2004-27 and G.99-103) had good values for the investigated parameters. The work adds to our knowledge of genotype-screening for drought resistance as an effective technique for choosing materials for advanced breeding programs, especially in controlled drought environments. More genomic and molecular research is required to comprehend the precise processes behind sugarcane drought resilience.