Sustainable clean production of soy bean in sandy soils be in need of biofertilizers efficient to reduce or replace mineral fertilizers that have environmentally unfriendly effects on soil, plants, environment and human health. The study conducted to investigate the effects of some biofertilizers individually or in combination. Bradyrhizobium japonicum was used in all treatments, Azotobacter chroococcum, Bacillus megterium, and arbuscular mycorrhizal fungi (AMF) were used individually or in a mixture of all, with or without a nutrients solution as mixture of 2% humic acid and marine algae extract. The application of biofertilizers improved soil biota, increased Azotobacter and Bradyrhizobium count and plant height, shoot dry weight, root dry weight, number of branches, number and dry weight of nodules, nitrogen content and ethylene production of nodules, dry weight of seeds, total lipids, total saturated fatty acids, total sugars and total carbohydrates over the control. Adding nutrients to the microbial treatments had no positive effects on total microbial count (except the mixture with nutrient treatment), phosphate dissolving bacteria (PDB), Bradyrhizobium, plant height, shoot dry weight, root dry weight, number and dry weight of nodules, nitrogen content of nodules, nodules ethylene production, total saturated fatty acids and total carbohydrates. However, adding nutrients to the microbial treatment significantly increased number of branches, weight of seeds and total lipid and almost doubled the content of total unsaturated fatty acids over the control. The results could serve as a constructive approach, for supplementary research in the integrated plant-microbe interaction in agriculture.