This study examined the effects of foliar spray of amino acids and thiamin on the yield and quality of iceberg lettuce (Lactuca sativa) in the summers of 2020 and 2021 under the hydroponics cultivation method. Aviram and Sara, two hybrids, were raised. Five weeks of once-weekly applications of Aminototal (20% free amino acids) and thiamin at concentrations of 1, 2, and 3 cm³/L and 0.06, 0.08, and 0.1 g/L, respectively, were conducted. All foliar spray treatments enhanced plant growth when compared to the control. The N, P, K, Ca, and Mg mineral contents benefited from the given treatments. The Aviram hybrid marketable and total yield were most strongly influenced by the foliar spray treatment with the highest amino acid concentration. Contrarily, the control treatment generated the smallest total and marketable yield of lettuce heads (without amino acids and thiamin).