Date palm somatic embryogenesis has become an ideal technique for large scale production of distinguish and rare cultivars. Date palm cultivar, culture medium components and culture conditions have been identified as detrimental factors affecting somatic embryo formation process.  This research investigated the potential impact of ammonium nitrate (NH₄NO₃), calcium nitrate Ca(NO₃)₂ strengths and polyethylene glycol (PEG) concentrations on mature, germinated and vitrified embryos during maturation stage of cv. Medjool callus cultures. Also, the effect of calcium chloride (CaCl₂.2H₂O) levels that could improve somatic embryo germination (shoot formation) and secondary embryo numbers during germination stage was examined. Rooting was also determined as affected by phloroglucinol (PG) levels. The ideal amount of potassium sulphate (K₂SO₄) or aluminosilicate (Al4Si₄O₁₀ (OH)₈) (kaolin) as anti-transpirants that influenced the survival and growth of cv. Medjool plants was also investigated in relation to the ex vitro acclimatization conditions. Modification of Woody Plant Medium (WPM) maturation medium by using NH₄NO₃ at double strength and Ca(NO₃)₂ at fourth strength encouraged the highest significant value of mature embryos formation. NH₄NO₃, Ca(NO₃)₂ and PEG had a clear impact on mature, germinated and vitrified embryos during maturation stage of cv. Medjool callus cultures. Also, CaCl₂.2H₂O levels improved somatic embryo germination (shoot formation) and secondary embryo numbers during germination stage. PG in the rooting medium had a beneficial role in root system. Using Al₄Si₄O₁₀ (OH)₈ at 0.1 mg/l as an anti-transpirant material enhanced survival percentage of plant growth during ex vitro acclimatization stage.