This study aimed to contribute to the sustainable management of endemic fish species from the Glossogobius genus in Lake Towuti through conservation initiatives focused on domestication, particularly by investigating the reproductive characteristics of G. flavipinnis and G. intermedius. The research was conducted between June and September 2024 in Lake Towuti, South Sulawesi, with fish sampling taking place at three sites: Tanjung Timbala, Tanjung Lengkobutanga, and Tanjung Bakara. At Station 1 (Tanjung Timbala), the gonado somatic index (GSI) for male G. flavipinnis ranged from 0.22 to 1.40%, while for females, it varied from 0.22 to 2.18%. For G. intermedius, males exhibited a GSI ranging from 0.71 to 1.05%, and females from 0.97 to 1.68%. At Station 2 (Tanjung Lengkobutanga), GSI values for male G. flavipinnis ranged from 0.01 to 1.34%, and for females, from 0.01 to 2.40%. In G. intermedius, male GSI values were between 0.93 and 1.16%, while females ranged from 0.99 to 1.31%. At Station 3 (Tanjung Bakara), the GSI for male G. flavipinnis varied between 0.01 and 1.76%, with females ranging from 0.01 to 2.40%. The fecundity of G. flavipinnis was recorded to be between 54 and 130 eggs, whereas G. intermedius produced between 75 and 138 eggs. The diameter of G. flavipinnis eggs across all stations in Lake Towuti ranged from 0.03 to 0.23mm, with the highest frequency for GML III found between 0.06 and 0.08mm, and for GML IV between 0.18 and 0.20mm. In contrast, the egg diameter for G. intermedius ranged from 0.03 to 1.22mm, with the most frequent size for GML III between 0.03 and 0.17mm, and for GML IV between 0.93 and 1.07mm.