In this study, the impact of light-emitting diodes (LED) on cultivation of the microalga Chlorella ellipsoidea within a bioreactor system was analyzed. The study applied two different illumination systems, a tank illuminated with natural light (CT1 group) and a tank illuminated using LEDs (CT2 group). Statistical analysis revealed no significant difference (P>0.05) in the cell counts between CT1 and CT2 groups over the cultivation period, with maximum cell densities of 7.0 x 10⁷ and 8.8 x 10⁷ cells mL^-1, respectively. The cell size and amino acid content of C. ellipsoidea did not differ significantly between the groups. Fatty acids predominantly comprised linoleic acid and oleic acid, with significantly (P<0.05) higher quantities observed in the CT2 group. To evaluate the transfer of nutrients to zooplankton, C. ellipsoidea from the two groups were fed to Moina sp. (CT1 → MT1, CT2 → MT2). After two days, both Moina sp. groups had similar protein levels (64%), but they differed in fat content: 3.41% in MT1 and 6.90% in MT2. In summary, the cultivation of C. ellipsoidea under blue LED light resulted in higher levels of fat and fatty acids compared to cultivation under natural light. Additionally, this system was effective in transmitting the nutritional value to zooplankton. In conclusion, the results indicate that bioreactor tanks with blue LED have the potential to produce C. ellipsoidea and Moina sp. suitable for small-scale aquaculture and ornamental fish farmers. However, the choice of the LED cultivation system should be made judiciously, considering its optimal utilization during periods of low natural light to achieve the best cultivation results.