Wound infections caused by multidrug-resistant (MDR) bacteria are a significant concern due to the high morbidity and mortality rates. This study aimed to evaluate the antibacterial activity of Arthrospira platensis extract against MDR bacteria that cause wound infections. Antibacterial activity was evaluated by determining the values of minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) with the microdilution method. The results from the study showed that A. platensis extract has antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA), MDR Enterococcus faecalis, MDR Escherichia coli, MDR Klebsiella pneumonia, and MDR Pseudomonas aeruginosa. In this study, the MIC and MBC values of each A. platensis extract were ≥6.25mg/ mL. A. platensis has the potential to be developed as an antibacterial agent. To further explain the antibacterial activities of A. platensis extracts, more in vivo research and the identification of mechanisms of action are necessary.