Antibacterial Activity of Chlorophyll c from Sargassum polycytum against Methicillin-Resistant Staphylococcus aureus (MRSA) and Multidrug-Resistant Escherichia coli (MDR E.coli) - Egyptian Knowledge Bank

395116

Antibacterial Activity of Chlorophyll c from Sargassum polycytum against Methicillin-Resistant Staphylococcus aureus (MRSA) and Multidrug-Resistant Escherichia coli (MDR E.coli)

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Last updated: 23 Dec 2024

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Abstract

In this study, chlorophyll c, extracted from Sargassum polycystum using a rapid maceration method, was investigated for its antibacterial properties against multi-drug resistant (MDR) E. coli and methicillin-resistant Staphylococcus aureus (MRSA). The extract was fractionated with diethyl ether, and chlorophyll c was isolated using preparative thin-layer chromatography. UV-Vis spectrophotometry and FTIR confirmed the identity of chlorophyll c, revealing functional groups such as aldehyde, ester, C-H, OH, and hydroxyl groups. The antibacterial activity was tested using the agar well diffusion method. Against MRSA, chlorophyll c showed an inhibition zone of 10.13mm at 0.2%, increasing to 11.25 mm at 0.4%, compared to ciprofloxacin's 13.34 mm. For MDR E. coli, the inhibition zones were smaller, with 7.93 mm at 0.2%, 8.99mm at 0.3%, and 9.86mm at 0.4%, compared to 10.21mm for ciprofloxacin. The results suggest chlorophyll c exhibits greater antibacterial activity against MRSA than MDR E. coli, highlighting its potential as a natural antibacterial agent.

DOI

10.21608/ejabf.2024.395116

Keywords

Antibacterial activity, Chlorophyll c, isolation, MRSA, MDR E.coli

Authors

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First Name

Kusmita

Last Name

et al.

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Affiliation

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City

Orcid

Volume

Article Issue

Related Issue

50139

Issue Date

2024-09-01

Receive Date

2024-12-04

Publish Date

2024-09-01

Page Start

959

Page End

970

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 28_Issue 5_Pages 959-970.pdf

PDF . 343.7kB

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https://ejabf.journals.ekb.eg/article_395116.html

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https://ejabf.journals.ekb.eg/service?article_code=395116

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133

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Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

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https://ejabf.journals.ekb.eg/

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