An essential cultural parameter that influences the growth, reproduction, and physiological responses of fish species is stocking density. Thus, the goal of this research was to determine the effects of population density on water quality, growth activity, and reproduction of L. ramada in captivity. This investigation examined blood ion levels in L. ramada broodstock at varying stocking densities, along with albumin, total protein, and hormones related to gonadal maturation and growth. Ponds with different densities showed no significant differences in water quality. Growth hormone (GH) concentrations increased during gonadal maturity in ponds with low densities, while they decreased in ponds with high densities. Total protein and various serum ion concentrations were similar to GH levels, revealing a notable increase at low density compared to high density, with mature fish exhibiting higher levels. Additionally, the values of albumin and the albumin/globulin ratio changed only slightly across different densities and throughout maturation. High growth rates in total length and weight were observed in L. ramada stocked at reduced densities, along with higher values for condition factor and survival rate. Conversely, the food conversion ratio was lower in L. ramada raised in low-density ponds. However, broodstock of L. ramada reared at minimal density showed an increased sexual activity. Histological examinations of the testes and ovaries revealed that fish stocked at reduced densities had the highest values for the gonadosomatic index (GSI) and hepatosomatic index (HSI). Moreover, a strong correlation was observed between the GSI cycle and the concentrations of estradiol (E2) and testosterone (T). Based on these findings, it was concluded that the reduced population density is beneficial for the growth and gonadal maturation of L. ramada.