This study examined the potential utilization of banana peel waste as a source of prebiotic oligosaccharides to improve the biological performance of the milkfish (Chanos chanos). A comprehensive study was conducted to evaluate the effect of banana peel oligosaccharide supplementation on feed digestibility, feed consumption, growth, and survival rate. The milkfish specimens were fed diets supplemented with banana peel oligosaccharide extract at levels of 0 (control), 1, 2, 3, and 4% (initial weight 15± 5g/ ind) maintained at a density of 10 fish per aquaria, measuring 50 × 40 × 35cm³, totaling 15 units. This study was designed in a completely randomized design (CRD) with five treatments and three replications. The experimental fish were fed with 5% of fish biomass per day for 50 days of rearing, with a frequency of feeding three times a day at 07:00, 12:00, and 17:00. The results showed that the oligosaccharide extract had a significant effect (P < 0.05) on digestibility (total, protein, fat, and energy) and growth (absolute length and relative length), while no effect was detected on the survival and feed consumption of the milkfish. The best dose varied depending on the parameters: 1% for fat digestibility (93.69 ± 0.76%), 2% for total digestibility (18.83 ± 0.19%), protein digestibility (92.58 ± 0.66%), energy digestibility (90.65 ± 1.23%), in addition to 3% for absolute length growth (11.04 ± 0.85cm) and relative length growth (8.29 ± 0.50%). In conclusion, the addition of banana peel oligosaccharide extract as a prebiotic improves the growth and nutritional performance of milkfish, with the best dose ranging from 2–3%, depending on the optimized parameters.   Banana peel, Digestibility, Feed, Oligosaccharide, Prebiotic