Salinity is a crucial environmental factor that influences the growth, reproduction, and physiological responses of fish species. Understanding how different salinities affect Liza ramada is essential for selecting optimal conditions for aquaculture. This study aimed to assess the effects of salinity on the growth, osmoregulation, stress response, and maturation of L. ramada in captivity. In this investigation, blood levels of hormones related to growth, adaptation, and stress response were measured, along with glucose levels in L. ramada broodstock raised in the waters of varying salinities. Results indicated that prolactin and growth hormone levels were higher in freshwater and lower in saline conditions. Additionally, these hormone levels increased during gonad maturation across all salinities. In contrast, thyroxine concentrations exhibited a pattern similar to cortisol, with significantly higher levels in saline water compared to freshwater. Mature fish also displayed elevated hormone levels. Glucose concentrations in the fish raised in different salinities followed a similar trend to cortisol levels. Furthermore, triiodothyronine hormone levels varied slightly during maturation and across different salinities. The L. ramada stock in freshwater demonstrated superior growth rates in both length and weight, alongside higher condition factor and survival rates. However, broodstock grown in freshwater had a lower food conversion ratio. In saline environments, L. ramada exhibited increased sexual activity, with a higher frequency of mature gonad stages and elevated values of the gonadosomatic index (GSI) and hepatosomatic index (HSI). In light of these findings, we conclude that freshwater is conducive to the growth of L. ramada, while saline water is essential for achieving sexual maturity.