Nannochloropsissp is used as natural feed since it contains high good nutrition for the growth of fish larvae. However, the availability of Nannochloropsis sp. is still limited and has become a constraint in aquaculture. This study aimed to address the effect of pasta on the density of Nannochloropsis sp. cultured on the laboratory scale from pasta product of massive culture using the combination of agricultural fertilizer and water extract of Moringaoleifera leaf. Nannochloropsis sp. culture of the laboratory scale used the kw21 fertilizer. The Nannochloropsis sp. seeds were taken from Nannocloropsis sp. pasta with different concentrations, while the control Nannocloropsissp seeds used the laboratory scale without pasta. The study applied a complete randomized design with 4 treatments (NaOH pasta of 75 ppm, 100 ppm, 125 ppm, and control) and 3 replications. The control treatment (D) used the Nannochloropsis sp. of the laboratory scale without pasta. Data analysis utilized the one way-ANOVA, followed by the least significant difference test upon detecting a significant difference. Results indicated the significant effect of the NaOH pasta on Nannochloropsis sp. density.