This study was conducted in Nineveh Governorate, Iraq, where four different sites were identified. Plants in these sites receive water from various sources, including domestic sewage, industrial wastewater, and waste from electric generators. These sites are Wadi Aqab Industrial Area, Al-Khosr, the Old Bridge, and Al-Faisalia (control). The aim of this study was to determine how polluted water from different sources affect the chemical composition of selected wild plants, namely Silybum marianum and Xanthium strumarium L. The results showed that the amino acids most affected in Silybum marianum by polluted water are Serine and Glycine, with concentrations of 389 and 317mg/l, respectively, in the Al-Khosr area, compared to other amino acids in the study areas. For Xanthium strumarium, the results indicated that the amino acid most affected by polluted water is Histidine, with a concentration of 198mg/l in the Old Bridge area, compared to other amino acids in the study areas.