This study was set out to quantify biochemical parameters in freshwater prawns (M. nipponense) raised in four different salinity levels and temperature ranges (20, 25, 30, and 35°C) as well as stress enzymes (ALT and AST), ionic content (Cl, K, Na, and Ca), and osmotic pressure. The Shatt al-Arab River in the Al-Mashab region of southern Iraq was the site of the M. nipponense sample collection. This study used a recycling aquarium system (RAS) with sixty-liter plastic containers for each subject. In comparison with the control group, all metrics increased significantly (P< 0.05), although biochemical parameters decreased significantly (P< 0.05). In addition, a negative relationship was detected between temperature, salinity, total protein, glucose, K, Na, ALT, and AST and a positive relationship with urea, Cl, Ca, and cholestrol.