A study on the population dynamics of Marble Goby (Oxyeleotris marmorata, Bleeker, 1852) was conducted to support the conservation and sustainability in Huai Kho Reservoir. Fishermen were instructed to set fishing nets monthly from January to December 2022. This study successfully collected 284 fish specimens, with lengths ranging from 115 to 236mm and an average length of 162.97± 24.60mm. The total weight ranged from 24 to 236g, with an average weight of 60.87± 31.00g. The growth performance index (ϕ) was calculated to be 3.986 per year, with an asymptotic length (L_∞) of 273.00mm, and a growth coefficient (K) of 0.13. The probability of capture was determined to be L25%= 137.18mm, L50%= 144.90mm, and L75%= 163.02mm. The length-weight relationship (LWR) was found to be W= 0.02443L^1.61202 (r² = 0.92). The rate of total mortality (Z) was 0.97 per year, natural mortality (M) was 0.26 per year, and fishing mortality (F) was 0.71 per year. The highest recruitment (maximum replacement rate) was observed annually from March to June, ranging from 11.63 to 18.82%.  The total steady-state biomass was calculated to be 6.50 tons, with the current exploitation rate (E) rate being 0.73 (E>0.5). The results indicate that the marble goby stock was overexploited