The tiger shrimp (Penaeus monodon Fabicius, 1798) is a popular Indonesian fishery product with high market value both domestically and internationally. Its high nutritional value makes it a valuable product that is in high demand. Along with increased production, various challenges appeared in management and feed quality aspects. Multi-enzymes are one method for increasing the tiger shrimp productivity. This study aimed to identify the dose of multi-enzymes in the feed that leads to an optimal growth performance, and optimum activity of digestive enzymes (amylase, protease, lipase), as well as improving the essential amino acids index in the shrimp body. This study used a completely randomized design (CRD) with 4 treatments and 3 replications each, thus 12 experimental units were applied. The treatments used were A at 0%/kg feed, B at 40%, C at 60%, and D at 80%/kg feed. The experimental animal was the tiger shrimp with a mean weight of 0.07g/ ind. The shrimps were reared in the hatchery laboratorium of the Marine Science and Fishery Faculty, Hasanuddin University, Makassar. The parameters examined were essential amino acid index (EAAI), essential amino acid score (EAAS), enzyme type activity, specific growth rate (SGR), relative weight gain (RWG), survival rate (SR), and protein efficiency ratio (PER). The results showed that feed with 80% multienzyme/kg provided the highest EAAI compared to other doses, namely 2.64. In addition, the most EAAS deficiency occurred in methionine EAA, namely 9.29 in multi-enzyme feed with a dose of 40%/kg. SGR, RWG, and PER were not significantly different (P> 0.05), but SR showed a significant difference (P< 0.05). The activity of amylase, protease, and lipase enzymes in the tiger shrimp also increased with the addition of multienzyme doses to the feed.