The development of a checklist of non-native aquatic plants in the Brantas River is very important from an ecological perspective. The checklist of non-native aquatic plants provides valuable information for the development of effective management and mitigation strategies. The main objective of this study was to provide a detailed checklist of non-native aquatic plants present in the Brantas River, East Java, Indonesia.  Sampling was conducted at several locations along the river, where site selection included differences in habitat and environmental conditions, from Blitar (upstream), Trenggalek (midstream), Kediri (midstream), and Jombang (downstream). At each selected site, five 1 x 1m quadrats were randomly placed along the riverbank and within the waterbody using a stratified random allocation within each habitat type (shallow area, slow-flowing section, tributary mouth). Comprehensive identification of physiological and ecological characteristics of non-native species was based on published books and scientific journals. The result of this research showed that there are several non native invasive aquatic plants in Brantas River, viz.  the alligator weed, Ludwigia, water lettuce, floating fern, frogbit, parrot feather, Hydrilla, Ambulia, Lemna, water chestnut, Cabomba, and the water hyacinth, Azolla. We recommend management control through mecanical, biological and chemical alternative.