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367988

Genotoxicity of Chlorophyllin Compared with Other Pesticides Used to Control Culex pipiens Larvae (Diptera: Culicidae)

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Last updated: 01 Jan 2025

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Abstract

Mosquitoes are prime examples of vectors for various diseases such as malaria, the West Nile virus, elephantiasis, dengue fever, and yellow fever. The repeated use of chemical pesticides has created numerous obstacles and environmental risks including mosquito resistance to insecticides. This work aimed at assessing the larvicidal effect of chlorophyllin (a water-soluble substance obtained after removing the phytol tail from chlorophyll) and coumarin as photosensitizers, compared to the microbial insecticide Bacillus thuringiensis israelensis (Bti) and permethrin, a chemical insecticide, against the third larval instar of Culex pipiens larvae monitoring its mode of action and its genotoxic effect. Photosensitizers exposed to sunlight generate reactive oxygen species (ROS), with singlet oxygen (1O2) capable of killing parasitic organisms primarily in aquatic systems. Our experiments demonstrated a high potential activity of Na-Cu chlorophyllin against mosquito larvae after at least 8 hours of incubation in the dark. The LC50 values were 0.22x10-3, 0.96x10-2, 13.7x10-2, and 4.59x10-1 mg/ l after 24 hours of exposure to chlorophyllin, Bti, permethrin, and coumarin, respectively. The molecular changes after treatment with chlorophyllin were tracked using RAPD-PCR with six arbitrary DNA primers. Results confirmed no significant changes or genetic damage after treatment with chlorophyll derivatives.

DOI

10.21608/ejabf.2024.367988

Keywords

Photosensitizers, chlorophyllin, Coumarin, permethrin, genotoxicity, Mosquito

Authors

First Name

Hadeer M.

Last Name

Elshemy et al.

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Orcid

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Volume

28

Article Issue

4

Related Issue

48991

Issue Date

2024-07-01

Receive Date

2024-07-18

Publish Date

2024-07-01

Page Start

297

Page End

311

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 28_Issue 4_Pages 297-311.pdf

PDF . 778.9kB

Link

https://ejabf.journals.ekb.eg/article_367988.html

Detail API

https://ejabf.journals.ekb.eg/service?article_code=367988

Order

18

Type

Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Genotoxicity of Chlorophyllin Compared with Other Pesticides Used to Control Culex pipiens Larvae (Diptera: Culicidae)

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Type

Article

Created At

23 Dec 2024