The goal of this investigation was to examine the occurrence and virulence features of the antibiotic-resistant fish-borne Aeromonas hydrophila that were isolated from fish sold in Aswan City, Egypt. A total of 125 fish samples (25 of each:the mMullet, the sSaurus, the cCatfish, the Nile perch, and the Nile tilapia) were examined. The mean Aeromonas counts in the examined samples varied from 3.89± 0.35 logCFU/g in the Nile tilapia to 2.68± 0.13 log CFU/g in the Nile perch. 35.2% of the inspected samples had Aeromonas spp. and A.hydrophila was as the majority, then followed byA. sobria (11%), A. caviae (4%), and A.veronii (3.2%). Based on the species-specific 16S rRNA gene, it was found that 16 isolates were confirmed as A. hydrophila and possessed 62.5%, 25%, and 37.5% of the virulence genes for aerA, ahh1, and altA, respectively. Additionally, A. hydrophila exhibits varying levels of resistance to the most extensively verified antibacterial substances. SoTherefore, it is recommended that hygienic techniques be used upon handling, preparing, processing, and storing fish to improve its quality and reduce microbiological contamination with A.hydrophila. Additionally, it is crucial to motivate researchers to pursue non-antibiotic control strategies for this type of pathogen and other bacterial infections in farmed fish.