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365569

Biodegradation of Low-Density Polyethylene Plastic Using Marine Bacterial Consortium

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Last updated: 23 Dec 2024

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Abstract

Recently, plastic wastes are considered a main environmental problem, and many bacterial isolates were tested to biodegrade them. The low-density polyethylene (LDPE) plastic sheets were tested to be degraded by a marine bacterial consortium. The potent marine plastic degrading isolates were biochemically identified as Bacillus licheniformis, Bacillus subtilis, and Paenibacillus xylanilyticus using the BIOLOG identification system. The identification of the most potent plastic-degrading bacterium was confirmed as Bacillus licheniformis FMMA using the 16S rRNA gene sequence. This bacterial consortium was physiologically adjusted as follows: pH 7, temperature 35°C, inoculum size 4ml/ 100ml (1.0X107CFU/ ml), and an incubation period of 30 days. It led to 34.1% plastic loss of weight. The mechanical properties (maximum force and the elongation% at break) of these treated LDPE plastic sheets showed 7.49N and 112.2%, respectively, compared to that of B. licheniformis FMMA, which showed a 25.5% plastic weight loss, with maximum force and elongation% at a breakpoint of 8.9N and 114.2%, respectively. In addition, the plastic biodegradation was also estimated through a scanning electron microscopy and Fourier transform-infrared (FTIR) spectroscopy, were a great reduction in the intensity of the -CH2 peak appeared at 2900cm-1, and the disappearance of the -OH peak at 3500cm-1 was observed. 

DOI

10.21608/ejabf.2024.365569

Keywords

Marine bacteria, biodegradation, LDPE plastic, Bacillus licheniformis, B. subtilis, Paenibacillus xylanilyticus

Authors

First Name

Manal M.

Last Name

El-Naggar et al.

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Volume

28

Article Issue

4

Related Issue

48991

Issue Date

2024-07-01

Receive Date

2024-07-07

Publish Date

2024-07-01

Page Start

1

Page End

21

Print ISSN

1110-6131

Online ISSN

2536-9814

Link

https://ejabf.journals.ekb.eg/article_365569.html

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https://ejabf.journals.ekb.eg/service?article_code=365569

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Biodegradation of Low-Density Polyethylene Plastic Using Marine Bacterial Consortium

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Article

Created At

23 Dec 2024