Pterygoplichthys pardalis is an invasive fish species that grows rapidly in Lake Tempe, Kabupaten Wajo, and has disturbed the existence of native, endemic, and introduced fish. This study aimed to analyze the reproductive biology of this species in terms of population control. The research was conducted from January to April 2023 at Tempe Lake, Wajo Regency, South Sulawesi. Fish samples were caught using gill nets, cleaned, and stored in a coolbox for observation in the laboratory. Reproductive biology observations began with the separation of male and female fish, measuring the length and weight of each with a fish measuring board and analytical scales. Samples were dissected for maturity gonad and fecundity observations. Length-frequency distribution of male fish ranged from10.08- 41.58cm, while that of female fish ranged from 10.08- 35.28cm. The overall sex ratio between male and female fish was 1:1 (balanced), while the sex ratio between mature males and females (maturity stage IV) was 1:2 (polygamy). The growth pattern exhibited a negative allometric trend, with a long spawning season reaching its peak in March. The species is considered a partial spawner.The length at first maturity of gonads (L_m) for male and female fish were 28,99 and 25.98cm,  respectively. Moreover, the fecundity ranged from 201– 5,050 eggs with an average of 1,762± 966 eggs.