This study aimed to isolate and comprehensively characterize chitinase-producing bacterial isolates collected from diverse aquatic stations in the Red Sea. Out of twenty nine isolates, chitinase-producing marine Enterococcus hirae (OR064172) and Enterococcus faecalis (OR053873) were biochemically and genetically identified. The two isolates have primary specific activity of 10.7 and 11.5U/ mg for E. hirae and E. faecalis, respectively. To enhance the purity of the chitinase enzymes, ammonium sulfate precipitation was employed, yielding 12.9 and 13.47U/ mg for 70% fractions of E. hirae and E. faecalis, respectively. Afterward, DEAE-cellulose chromatography was utilized for the purification process, resulting in two peaks for E. hirae and one peak for E. faecalis. The results of chitinase characterization indicated optimal conditions with a temperature of 37°C, a pH of 6.5, and a salt tolerance of up to 1% for both strains. The in-depth kinetic chitinase analysis unveiled the optimum K_m (0.03 and 0.07) and V_max (0.124 and 0.0003) conditions for chitinase activity for both E. hirae and E. faecalis, respectively. The application of the previous strains at the economic level involves the production of chitinase using marine shrimp waste in an attempt to extract beneficial products. The solid state fermentation process of this waste for both isolates was also tested, with results indicating that the specific activity of the produced enzyme was 8.48 and 11.38U/ mg, respectively, achieving the highest production after 48 hours. This study adeptly accomplished the isolation and identification of chitinase-producing marine bacterial isolates. The rigorous purification and comprehensive characterization of chitinase enzymes offer valuable insights into their production, refinement, and utilization across diverse industries, particularly within aquaculture and waste management.