This work aimed to study the potential of aquaculture wastewater (AWW) as a nutrient source for Chlamydomonas reinhardtii, Chlorella vulgaris,and Haematococcus lacustris cultivation. Investigating nutrient removal for these microalgae was evaluated. The best phytoremediation was observed by C. reinhardtii with removal efficiencies of 82 and 62.3% for nitrate and chemical oxygen demand (COD), respectively, while all strains were able to remove 100% of total phosphorus (TP). Although the aquaculture wastewater (AWW) was a suitable medium for microalgae, the 3 microalgae grew faster with more biomass on BG11 than on AWW. In AWW, nutrients were lower than in BG11, which may contribute to microalgae stress; this stress enhanced lipid and carbohydrate production compared to BG11. C. reinhardtii exhibited the highest biomass of 1.00±0.07mg/L in AWW and the highest Diphenylpicrylhydrazyl (DPPH) scavenging activity of 63.08±2.09%. These findings demonstrate the potential of microalgae to remove and utilize nutrients in AWW while achieving valuable biomass productivity.