The use of natural materials in modern times is one of the interests of science. In recent years, there has been a rapid and exciting development in the use of biologically- based organic materials, for example, innovative drug treatments, with a focus on addressing, diabetic wound healing, cancer, and infectious diseases.  Focus on tissue responses, chitosan has been registered as a representative lightweight organic material for its distinctive properties, such as formability, processability, non-toxicity, and chemical activity, in addition to its compatibility with chemical flame and chemical sensitivity. Most importantly, it has antimicrobial activity and promotes wound healing. In this work, the applications of chitosan were used as a wound dressing. Optimized chitosan was estimated for wound healing through topical cream application for three successive days on surgical cuts made in (Lumbricus castaneus) earthworms, as an alternative for human skin. The outcomes showed conspicuous decreases in both wound closure and re-epithelialization duration in chitosan-treated earthworms as evident through macroscopic examination and thorough histological scrutiny utilizing light and electron microscopy, in comparison with the control group.  In summation, these findings posit that a wound dressing in chitosan holds promise as a catalyst for wound healing. Future endeavors will delve into scrutinizing the intricate mechanisms underlying this influential phenomenon.