Microalgae have attracted particular interest since they represent a rich biological resource for many applications and one of the most promising natural sources of zeaxanthin, which is used in a wide range of pharmaceutical and food applications. Environmental factors such as light affect the production and accumulation of zeaxanthin in cyanobacteria. This study investigated the effect of different light intensities (40, 60, 80, 100 and 120 µmol m-2 s-1) on growth rate and zeaxanthin production in the cyanobacterium O. amoena. The results of zeaxanthin production by O. amoena showed that the highest value of zeaxanthin was 0.9390µg/ mg, which was recorded at a light intensity of 80µmol m-2 s-1, while the lowest value (0.0807µg/ mg) was recorded at 40µmol m-2 s-1. Based on the research results, it can be deduced that O. amoena shows a good production of zeaxanthin upon exposure to an appropriate light intensity. While statistical analysis showed no differences in zeaxanthin production among the light intensities used in the current study at P> 0.05.