A total of 150 fish samples were obtained from two sources: the local fish markets, for specifically the Nile tilapia (Oreochromis niloticus) specimens in addition to the African catfish (Clarias gariepinus), with 25 samples collected for each species. The second source is the farms raising fish for export, including meager (Argyromus regiym), the European sea bass (Dicentrarchus labrax), the European eel (Anguilla anguilla), and flathead grey mullet (Mugil cephalus), with 25 samples for each species. The data collection process involved the use of the fundamental random sampling methodology. The sampling was conducted between November 2022 and July 2023 addressing various aquaculture sites in Kafr Elshiekh Governorate, Egypt. The samples were immediately transferred to the laboratory for antimicrobial screening using an icebox, without any delay. Antibiotic residues belonging to the B lactam group were identified in the Nile tilapia at a prevalence rate of 4.67% among the total sample size of one hundred and fifty fish specimens. No traces of other antibiotic residues, including tetracycline, aminoglycosides, fluoroquinolones, and macrolides, were found in the samples of the Nile tilapia. The presence of all chosen categories of antibiotics was not seen in any of the surveyed samples, which included African catfish, meager, flathead grey mullet, European eel, and European sea bass. In conclusion, the current findings indicated that 4.67% (seven samples) of the surveyed samples contained detectable antibiotic residues, while 95.33% (143 samples) did not show any trace of antibiotic residues.