Lake Nasser is the main water resource in Egypt, thus continuous evaluation of Lake Nasser is very important to avoid any problems at an appropriate time. In this study, bacterial loads of water, sediments, and plants were estimated in the northern Lake Nasser Khors (El-Ramla, Rahma, Kalabsha, and Wadi Abyad) to evaluate the quality of the lake. Results showed that the bacterial loads increased the inlet khor, especially in El-Ramla and Rahma Khors, hence the inlet khors are considered more suitable for living fish. Wadi abyad Khor recorded higher numbers of all bacterial indicators of pollution; this may be due to the increasing tourism ships south of the Lake to visit Abu Simbel Temple, observe migratory birds, and transport ships to Sudan. The Egyptian government is concerned with implementing an effective fisheries policy and an effective pollution control policy. This is evidenced by the comparison between this study (2022) and our previous study (2015). The comparison revealed a percentage increase of less than 25% for the various bacterial groups, with a decrease of more than 100% for bacterial indicators of pollution; this indicates some improvement. But it is still exposed to pollution, hence the laws and rules must be followed strictly for tourism ships and fishermen during their work in the lake and residence around the lake, as well as rationing the coastal agriculture around Lake Nasser.