Erythrina variegate (EV) possesses a wide range of biological activities, including-anticancer,-antidiabetic, hepatoprotective, -antirheumatic, antibacterial, and antiviral properties. Methanolic extract of Erythrina variegate (MetEEV) has not been studied for its neuroprotective properties.  The purpose of this study was to look into the effect of MetEEV-on-scopolamine (Sco)-induced anxiety, amnesia, and brain oxidative stress in zebrafish, in addition to figuring out the underlying mechanism. The zebrafish were given MetEEV (260, 520, and 780g/ L) for seven days in a row before being given Sco (100μM) for 30 minutes before the behavioral tests (locomotor activity and cognitive function). The in-vivo antioxidant status and Acetylcholinesterase- (AChE) -activity were also -evaluated for the further assessment of possible mechanisms of MetEEV in enhancing memory capacities in zebrafish. MetEEV-could-improve-memory-and-reduce-brain oxidative stress in sco-induced zebrafish, as well as regulating cholinergic function by inhibiting AChE activity. Our findings suggest that MetEEV could be a promising candidate compound for treating amnesia by restoring cholinergic activity and alleviating brain oxidative stress.