This study was conducted at eight different sites along the Nile River. Various fishing areas, including islands, canals, and bridge areas suitable for fishing with small boats or traditional hooks,  were investigated. This study determined whether these areas were suitable or not for aquatic life by assessing the physicochemical variables and water quality index (WQI) of the Nile River water. It is also possible to predict pollution in these areas by using these variables with the water quality index. The investigation was conducted between 2020 and 2021. The obtained values of physical and chemical variables of the Nile River water for the investigated sites were compared with the standard values for aquatic life published in the Canadian Water Quality Guidelines (2011). Based on the results, some variables examined had values that are higher than the recommended standards, affecting aquatic life in these areas. A higher concentration of physical and chemical parameters was observed during winter compared to summer. The COD/BOD ratio of the Nile River water in the investigated areas ranged from 3.89 in the summer season to 16.58 in winter. Its annual average was 10.24. The mean Mg2+ concentration in the investigated areas was 35.21mg/ l in winter and 31.26mg/ l in summer. NO₃ concentrations ranged from 61.12 to 724.58g/ l. The WQI was analyzed for 40 samples, of which 37.5% fall under grade B (good), 37.5% under grade C (poor), and 25%  under grade D (very poor). The values of the WQI ranged from 34.34 to 96.63 in terms of compliance with guidelines for the protection of aquatic life. Assessment studies should be annually conducted to determine the water quality status of the Nile to improve its suitability for aquatic life.