In the present investigation, we endeavored to elucidate the ramifications of substituting fishmeal (FM) with fiber-devoid pea peels protein source with concentrations of 15 and 25% on various physiological parameters of the Nile tilapia, Oreochromis niloticus. A total of 120 O. niloticus fingerlings were meticulously categorized into three distinct quartile replicates, each encompassing 10 piscine subjects within a 50-liter aquarium setting. The dietary regimens administered during this experimental phase encompassed: A control group devoid of any FM substitution, T15 featuring a 15% pea meal concentration and T25 with a 25% concentration. The data presented here indicate that varying amounts of pea byproduct in the diet of Oreochromis niloticus had no noticeable effect on water quality variables. Treatment groups (T15 and T25, fed with 15 and 25% pea byproduct, respectively) had considerably better growth performance characteristics than the control group, including final weight and weight increase. However, there was no statistically significant difference between the values of DWG and SGR (daily weight gain). The values of food conversion ratio (FCR), feeding efficiency (FE) and protein efficiency ratio (PER) were significantly varied at partial replacement of fishmeal by 15% pea byproduct (T15) compared to the control group. The hematological parameters and the proximate body composition showed no significant differences across all groups. These results intimated that the inclusion of pea meals as an alternative protein source can be seamlessly integrated into the dietary regimen of the Nile tilapia without engendering adverse physiological consequences. Such revelations offer invaluable academic insights into the potential incorporation of plant-derived protein sources in aquaculture, thereby proffering implications for sustainable pisciculture methodologies.