In this study, the quality and safety were addressed for tilapia fish cultured in two fish farms around Lake Qaroun which is irrigated with untreated agricultural drainage water from El-Battas and El-Wadi drains. The chemical composition, quality parameters, heavy metals (HMs), organochlorine pesticides (OCPs) and bacteriological were assessed and compared with the permitted Egyptian and International borders. A high rate of lipid was administered for tilapia cultured in farm 1, while tilapia fish of farm 2 exhibited high levels of protein and energy. Compared to the species under study in farm 2, the values of TVB-N, TMA-N, TBA and pH were higher for tilapia raised in farm 1. In addition, values of HMs (lead, cadmium, zinc and manganese) and OCPs were higher in the tilapia fish of farm 1 compared to those of farm 2. Total bacterial count and total coliforms were 3.85 and 1.60 (cell×103cfu/g) for tilapia farm 1, respectively; whereas, values of 3.22 and 1.10 (cell ×103cfu/g) were recorded for tilapia fish of farm 2. For the two farms under investigation, no staphylococcus or salmonella bacteria were detected in tilapia. Generally, values of freshness and quality, HMs, OCPs and bacteriological aspects in tilapia from the two farms were below the Egyptian and International maximum permissible limits (MPLs). Therefore, these fish are suitable and safe for human consumption. Accordingly, the quality and safety of fish in aquaculture is in the interest of public health.