Polychlorinated Biphenyls(PCB) contamination has spread throughout the world due to its high stability and tendency to accumulate in the bodies of living organisms resulting in serious health effects on organisms. Water samples were collected from four stations along the southern part of the Tigris River during the period from autumn (2022) to summer (2023) to determine the concentrations of PCB compounds. Twelve compounds of PCBs were identified in the study areas; namely, PCB153, PCB149, PCB141, PCB138, PCB101, PCB52, PCB44, PCB31, PCB28, PCB18, PCB194, PCB189 one dioxin-like PCB compound appeared (PCB189). The concentrations of total PCBs in water samples ranged from (1.2ng/L) during the winter to (18.8ng/ L) during the autumn season; these compounds recorded the highest level in the upper station compared to the lower stations. This may be due to human activities, household wastes and neighboring agricultural lands that use fertilizers and pesticides as sources of PCB compounds.