The provided list encompasses 43 fish species inhabiting the water bodies of the Chirchik River basin. These species are categorized into 8 orders, 15 families, and 38 genera. This comprehensive list includes all currently registered species whose validity has been revised in recent systematic revisions. The observations, conducted between 1996 and 2021, shed light on the ichthyological diversity along the Chirchik River. The compilation of species adheres to international ichthyological norms and reputable databases. Additionally, geographic coordinates were meticulously gathered and utilized to create a detailed Geographic Information System (GIS) map.