Trematodes are the most varied group of fish parasites. The present study investigated the trematode infection of fish collected from Port Said and IsmailiaGovernorate, Egypt during the period from March 2022 to February 2023. A total number of 360 fish of nine species were randomly collected. The parasitological examination showed that a total of 11 monogenetic trematodes were isolated from the gills, skin, and fins of different fish spp. (Cemocotyle carangis, Pricea multae, Furnestinia echeneis, Tetrancistrum indicum, paranella diplodae, Microcotyle chrysophrii, Calceostoma Calceostom and four different species of Diplectanum). In addition, four digenetic trematodes were isolated from the gastrointestinal tract of Dicentrarchus labrax (Acanthostomum spiniceps, Gonocerca trematomi, Erilepturus hamate, Metadena crassulata),andTransversotrema spp < em>. from the skin besides two encysted metacercaria from liver and muscle. Furthermore, Opisthadena spp. fromSardinella spp., Hexangium sigani from the gastrointestinal tract of Siganus revulatus, Clinostomum complanatum metacercaria from the branchial cavity of Oreochromis niloticus and  Tangiopsis chinensis from the gastrointestinal tract of Epinephelus aeneus were isolated. The present study revealed high parasite diversity which has dangerous impacts on fish.