This study aimed to investigate the acute toxicity (LC50) of a HEPA filter and its impact on the levels of reactive oxygen species (ROS) and heavy metal accumulation in common carp fish. In this study, carp observing approximately 8-9 cm in length were subjected to various concentrations of HEPA filters, such as 5%, 10%, 20%, 30%, and 40%. The findings of the study indicated that the utilization of a HEPA filter had an obvious effect on the levels of reactive oxygen species (ROS) in common carp fish. especially it was observed that the ROS level experienced a significant increase at a concentration of 40% (40 pg/mL). The concentrations of heavy metals Ag and Cu in the gill tissue exhibited a significant increase at a concentration of 40%, with values of 0.04 ppm and 0.013 ppm, respectively. In the gastric tissue, there was a notable increase in the concentration of heavy metals at 40% (approximately 0.015 ppm and 0.010 ppm, respectively).  The HEPA filter exhibits acute toxicity with an LC50 value of 39.65% concentration. In addition to this specific concentration, it has the potential to adversely impact the health of aquatic organisms at the tissue and organ levels.