Family Ampithoidae is considered one of the most famous and cosmopolitan amphipods worldwide since its early definition in 1888; however, information about this family from the Red Sea is still scarce so far. Hence, the purpose of this study was to revise all previously recorded and newly collected materials of Ampithoidae species in the Red Sea. Samples were collected for one year, beginning in the summer of 2019, from seven sites covering the Egyptian Red Sea coast from Hurghada to Halayeb with depths up to 35m. The results of this research revealed the species diversity of this family, which was represented by 8 genera and 9 species. The first evidence of the genus Biancolina Della Valle, 1893 was recorded from the Red Sea in addition to the recording of Cymadusa setosa Haswell, 1879, provided with the identification keys for each genus and species. This study concluded that the family Ampithoidae in the Red Sea is represented by 9 species: Amphithoides longicornis Kossmann, 1880; Ampithoe ramondi Audouin, 1826; Biancolina sp.; Cymadusa filose   Savigny, 1816; Cymadusa setosa Haswell, 1879; Paradusa bilobata Ruffo, 1969; Paragrubia vorax Chevreux, 1901; Pleonexes kava Myers, 1985 and Sunamphitoe falsa Barnard, 1932.