The present work was organized to explore the efficacy of chitosan (CS) and Moringa oleifera seeds (MOS) on heavy metal levels ((Pb) lead, (Cd), cadmium, (Cu) copper, (Zn) zinc, and (Fe) iron on five fish farms in the El-Fayoum Government. Heavy metal residues were analyzed in the fish muscle of Tilapia Nilotica, and water was collected from a commercial fish farm. The results demonstrated that iron was the most abundant metal in fish muscle and water samples from all farms. The highest levels were detected in fish and water from farm 2, with values of 1267.4±57.2 ppm and 3000±189 µg/l, respectively. Pb and Cd were not found in the Nile tilapia fish muscle. Our results revealed that metals were arranged in the following manner: iron, zinc, copper, lead, and cadmium for all farms, with farm 2 having the most significant quantities of heavy metals. Several chitosan and Moringa oliefera seeds (0.5 g/l, one g/l, and two g/l as powder and 1%, 2%, and 3% as extract) were used for water treatment. The highest efficient dosages of chitosan and Moringa oleifera seeds in heavy metal removal were two g/l as powder and 3% as an extract. Chitosan showed the highest cadmium removal rate (100%), whereas Moringa oliefera seeds recorded the highest Cd removal rate (79%). In conclusion, chitosan was a more efficient adsorbent for heavy metal elimination than Moringa. The highest elimination of metal ions from wastewater by chitosan powder 2g\l was 42.8, 100, 80.2, respectively, for lead, cadmium, and copper, while the highest elimination for zinc and iron was 62.54, and 55.9% by chitosan extract 3%.  Moringa seeds and chitosan had the following affinity for cations adsorbed selectively: Cd > Cu > Zn > Fe > Pb.