The current work aimed to study the phylogenetic lineages of some species of the genus Synodontis belonging to the family Mochokidae using the cytochrome c oxidase subunit I (COI) gene.  The nucleotide sequences of COI were inserted into the GenBank with accession numbers (OP143814.1-OP143816.1). The length of the cytochrome c oxidase subunit I sequence stretched from 617 bp to 623 bp. The overall genetic distance was 0.09. The COI gene displayed A+T  ratio bigger than the C+G ratio. The average nucleotide frequencies of adenine (A), thymine (T), cytosine (C) and guanine (G) were 27.30, 25.57, 29.93 and 17.20%, respectively. Among understudied Synodontis fish, the P-distances expanded from 0.0127 to 0.0161%. The highest value (0.0161) was found between Synodontis batensoda and Synodontis schall. While the lowest P-distance (0.0127) was found between Synodontis batensoda and Synodontis clarias. Furthermore, more investigations on the phylogenetics of the family Mochokidae are recommended.