Sixty freshly captured wild Trachurus indicus fish were randomly selected during the summer season of 2022 from the Suez Canal and examined clinically and bacteriologically to detect the most prevailing bacterial diseases affecting it.  Infected fish revealed hemorrhages at the operculum, base of the pectoral and pelvic fins and congestion in different internal organs. Motile Aeromonas septicemia and vibriosis were detected as the most prevalent and virulent bacteria isolated and identified by the traditional techniques; namely, Aeromonas hydrophila and Vibrio algynolyticus with a prevalence of 40% and 30%, respectively.  PCR was used for validation of the isolated pathogenic Aeromonas hydrophila and Vibrio species using the 16s rRNA gene and the virulence genes. All bacterial isolates were recovered from different internal organs and gills with the highest prevalence from the liver (45, 43.33 %)  followed by the kidney  (30, 33.33%), spleen (17.5, 16.67%), and gills  (7.5, 6.67 %), respectively.